Lumiscene Fr syns skull Transkript 1 Lumiscene Foumlr syns skull Marie Brigantini Polismyndigheten i Vaumlstra Goumltaland Specialarbete i Kriminalteknisk grundutbildning 2011 vid Statens kriminaltekniska laboratorium 2 Sammanfattning Utvecklingen av arbetsmetoder och utrustning inom det forensiska omraringdet garingr hela tiden framaringt. Preparar Lumiscene har utvecklats paring 2000-talet och lanserades paring marknaden Foumlr att visualisera smaring maumlngder latente blod anvaumlnds sedan naringgra aringr tillbaka bland annat preparatet Bluestar. Bluestar aumlr baserat paring luminol. Preparar Lumiscene aumlr hittills inte lika kaumlnt i Sverige. Lumiscene aumlr baserat paring baringde luminol och fluorescein, vilket enligt tillverkaren ger flera foumlrdelar. Arbetet syftar até att undersoumlka och presentera Lumiscene och i vissa avseenden staumllla det i jaumlmfoumlrelse mot Bluestar. Lumiscenes kaumlnslighet foumlr blod, specificitet foumlr blod och anvaumlndarvaumlnlighet har undersoumlkts. Lumiscenes paringverkan paring efterfoumlljande DNA-analyzer kommer kort att beskrivas. Undersoumlkningen visade bland annat att Lumiscene emitterade ett starkare ljus aumln Bluestar i kontakt med blod och att anvaumlndarvaumlnligheten var minst lika Deus som hos Bluestar 1. Alla bilder i arbetet, inklusive bilden paring framsidan, har tages av Marie Brigantini. 1 Foumlr foumlrklaringar av begrepp som har anvaumlnds sob denna rubrik haumlnvisas até texto sob rubtik 1 Syfte och sob rubrik 2 Bakgrund. I 3 Foumlrord Anders Nilsson paring biologienheten vid Statens kriminaltekniska laboratorium har varit faktahandledare foumlr det haumlr arbetet och Cecilia Ahlenius har varit spraringklig handledare. Jag vill tacka Anders Nilsson foumlr hans vaumlrdefulla raringd och dicas och svar paring allehanda fraringgor sob arbetets garingng och Cecilia Ahlenius foumlr hennes hjaumllp med den spraringkliga utformningen. Oumlvriga som boumlr naumlmnas med tacksamhet aumlr: - Karin Brandt och Natyra Bujupi paring Raumlttsmedicinalverket i Goumlteborg foumlr deras assistans paring laboratoriet med att blanda blodloumlsningar. - Helena Fabiansson paring tekniska rotelns fingeravtrycksgrupp i Goumlteborg, som varit bollplank i kemirelaterade fraringgor. - Fotograferna Anna Dahl och Anna Andreasson paring tekniska roteln i Goumlteborg samt fotografen Marcus Andrae paring Statens kriminaltekniska laboratorium som varit behjaumllpliga med sin erfarenhet angaringende fotografering av kemiluminiscens. - Mina gruppchefer, Carola Freudenthal och Oumlrjan Westerlund, um pouco mais e mais. ii 4 Inneharingllsfoumlrteckning av 1 syfte BAKGRUND Utveckling arbetsmetoder Luminol Fluoresceína Lumiscene material och metoder Lumiscenes kaumlnslighet foumlr blod jaumlmfoumlrt Med Bluestar Anvaumlnda preparat Preparering Utfoumlrande Specificitet Fotodokumentation ADN-analys Avgraumlnsningar Felkaumlllor Resultat Kaumlnslighet foumlr blod Specificitet ADN-analys Anvaumlndarvaumlnlighet Diskussion Kaumlnslighet foumlr blod Specificitet ADN-analys Anvaumlndarvaumlnlighet Foumlrslag até fortsatta undersoumlkningar Arbetsmiljouml och haumllsa Tid - och temperaturparingverkan paring preparates haringllbarhet Paringverkan paring efterfoumlljande foumlrproumlvningsmetoder Slutsats Kaumlllfoumlrteckning Litteratur Muntliga kaumlllor Internet Bilagor Bilaga Bilaga iii 5 1 syfte Undersoumlkningen har syftat até att: Undersoumlka Lumiscenes 2 kaumlnslighet foumlr blod. Undersoumlka Lumiscenes specificitet foumlr blod 3. Undersoumlka om Lumiscene har naringgon paringverkan paring efterfoumlljande DNAanalyser. Utoumlver detta kommer arbetet aumlven att beroumlra Lumiscenes anvaumlndarvaumlnlighet i faumlltarbetet samt dess kostnadseffektivitet. 2 Bakgrund 2.1 Utveckling av arbetsmetoder Vid brottsplatsundersoumlkningar efter grova varingldsbrott aumlr det inte ovanligt att brottsplatsen blivit uppsaringtligen staumldad eller tvaumlttad. Det oumlr heller inte ovanligt att viss tid hinner foumlrflyta mellan brott och brottsplatsundersoumlkning, ousado brottet inte upptaumlckts direkt eller daring brottsplatsen inte aumlr dado. Sob tiden hinner blodsparingr noumltas bort. Utvecklingen av arbetsmetoder och utrustning inom det forensiska omraringdet garingr hela tiden framaringt. Detençete decennet har det garingtt fortare framaringt aumln naringgonsin tidigare tack vare allmaumlnhetens intresse foumlr arbetet och flera privata foumlretags inmarsch paring omraringdet. Eu takt med denna utveckling oumlkar moumljligheterna foumlr oss brottsplatsundersoumlkare att saumlkra vaumlrdefulla kriminaltekniska sparingr. Men som med alla nya arbetsmetoder, o homem noggrant undersoumlka om kraven paring effektivitet, raumlttssaumlkerhet och prestation uppfylls. Inom kriminaltekniken har det utvecklats flera metoder foumlr att visualisera smaring maumlngder latent blod. Metoder har utvecklats sob 1900-talet som bygger paring luminol 4 eller fluorescein 5. Bluestar 6, som aumlr ett kommersiellt tablettbaserat kit de luminol, togs fram i boumlrjan av 2000-talet och kom smaringningom em paring den svenska marknaden runt Maringnga tekniska rotlar 2 Lumiscene, ett preparat foumlr visualisering e blod latente. Tillverkas av Loci Forensic Products, Nieuw-Vennep Holland. Utfoumlrligare information finns under rubrik Med specificitet menas hur blodspecifikt det aumlr, d v s om preparatet ger reaktion endast i kontakt med blod eller om reaktion erharinglls aumlven i kontakt med andra aumlmnen. 4 Luminol, en kemisk substans med det kemiska namnet Amino-2,3-di-hidro-1,4-ftalazindão. 5 Fluorescein, ett kraftigt faumlrgaumlmne med det kemiska namnet 6-hidroxi-9- (2-karboxifenil) - 3 (3H) - xantenon. 6 Bluestar aumlr ett utvecklat kit som bygger paring luminolmetoden homens daumlr den anvaumlnds i tablettform. Tillverkas av Bluestar Forensic, Monte-Carlo, Monaco. 1 6 runt om i landet anvaumlnder idag Bluestar 7. Lumiscene aumlr ett nytt tillskott paring omraringdet. Lumiscene har utvecklats i samarbete med den hollaumlndska kriminalpolisen och boumlrjade saumlljas internationalellt foumlr kriminaltekniskt bruk aringr Luminol Luminolbaserade loumlsningar reagerar med hemoglobinet i blodet. Hemoglobin aumlr ett protein i blodet som har till uppgift att transportera syre fraringn lungorna até kroppens alla vaumlvnader. Formato de formato de proteção i fyra kedjor, varje kedja inneharingller en hemgrupp. En hemgrupp aumlr en molekyl som i sin kaumlrna aumlr uppbyggd av kolvaumlten och som kan binda jaumlrnjoner. I varje hemgrupp finns en jaumlrnjon (Fe). En jon aumlr en molekyl som har underskott eller oumlverskott paring elektrisk laddning, eu det haumlr fallet har jonen underskott av negativa laddningar (dvs elektroner), então aumlr daumlrfoumlr positivt laddad. Under den kemiska reaktionen katalyserar hemoglobinet reaktionen mellan vaumlteperoxiden och luminolen, dvs det underlaumlttar reaktionen saringking kat ske utan att sjaumllv foumlrbrukas. Eu reaktionen antar luminolmolekylen en instabil form daumlr elektroner tillfaumllligt flyttar fraringn sina ursprungliga platser. Sob reaktionen oxideras 9 luminol i naumlrvaro av vaumlteperoxid, dvs det sker en kemisk reaktion med syret som finns bundet i vaumlteperoxiden, och luminol utsaumlnder ett blaringtt ljus, se foumlrenklad ilustração i figur 1. Detta fenomen, att ljus utsaumlnds vid den kemiska reaktionen, benaumlmns Kemiluminiscens. Ljuset uppstaringr ouy elektroner flyttar mellan olika elektronskal. Naumlr en elektron flyttar fringn ett skal med houmlgre energinivaring till ett skal med laumlgre energinivaring avges energi som vi uppfattar som ljus. Pesquisa por Foumlr att se reaktionen behoumlvs ingen extern ljuskaumllla men omraringdet kan behoumlva moumlrklaumlggas foumlr att kemiluminiscensen ska synas Vatten H2O Oxiderat reagens av luminol (3-aminophthalat) Figur 1 Foumlrenklad illustration oumlver luminols kemiska reaktion. Uppraumlttad av Marie Brigantini och Anders Nilsson. 7 Muntlig uppgift frank Christer Holmgren, Bluestar Escandinávia. 8 Muntlig uppgift fraringn Rene Gelderman och Martin Eversdijk, Loci Forensic Products. 9 Definição enligt Nationalencyklopedin Oxidation, kemisk reaktion vid vilken ett aumlmne avger en eller flera elektroner. 10 Delar av informationen stycket aumlr haumlmtad fraringn Chemiluminescens em química analítica, Ana M. Garcia-Campana, sid 7 2.3 Fluoresceína Fluoresceína aumlr ett faumlrgaumlmne som fluorescerar 11 i sig sjaumllvt utan kontakt med blod. Med hjaumllp av zink reduceras aumlmnet até fluorescência som inte fluorescência. Naumlr fluorescin kommer i kontakt med blod sker en reaktion, snarlik den som beskrivs i figur 1, Daumlr fluorescentes oxideras tillbaka até a fluoresceína fluoresceína formada. Fluoresceína fluorescente naumlr det belyses med ljus av 425 até 485 nm. Foumlr att se fluorescensen behoumlvs gul-eller filtro orangefaumlrgat Lumiscene Informationen om Lumiscene aumlr haumlmtad direkt fraringn tillverkaren Loci Forensic Products hemsida 13. Eftersom preparatet aumlr relativt nytt hittades i dagslaumlget ingen Annan Informationkaumllla. Lumiscene aumlr ett preparat som bygger paring en loumlsning av baringde luminol och fluorescein. Enligt tillverkaren ger kombinationen av luminol och fluorescein tre goda efekter, vilka beskrivs kortfattat i nedanstaringende stycken. Exakt vad som haumlnder i den kombinerade loumlsningen aumlr aumlnnu inte klarlagt. Lumiscene avger ett blaringgroumlnt ljus som aumlr laumlttare foumlr oumlgat att uppfatta aumln det blaringa ljus som avges vid luminolanvaumlndning. Intensiteten foumlr kemiluminiscensen aumlr houmlgre jaumlmfoumlrt med anvaumlndning av enbart luminol. Kemiluminiscensen fraringn Lumiscene emitterar ljus med varingglaumlngder kring 525 nm. I moumlrka foumlrharingllanden aumlr oumlgats celler som kaumlnsligast foumlr ljus med varingglaumlngder kring apenas 525 nm. Fluoresceinet i loumlsningen bidrar till en starkare kemiluminiscens. De flesta luminol - och fluoresceinloumlsningar inneharingller vaumlteperoxid. Vaumlteperoxid i foumlr houmlga koncentrationer kan ha en nedbrytande efekt paring DNA. Endast en laringg suspende vaumlteperoxid behoumlvs i Lumiscene. Lumiscene aktiveras med tvaring tabletter med avvaumlgd maumlngd vaumlteperoxid. Volymprocenten vaumlteperoxid i den faumlrdiga loumlsningen blir ca 0,12. Até a habilidade dos Lumiscenes laringga inneharingll av vaumlteperoxid naumlmns de vanliga luminol - och fluoresceinloumlsningarna med ca 3 vaumlteperoxid. Lumiscene aumlr i grunden en vattenbaserad produkt och salufoumlrs i tvaring olika varianter, Lumiscene och Lumiscene Ultra. Lumiscene Ultra aumlr en mer koncentrerad form de Lumiscene. Volymprocenten vaumlteperoxid aumlr enligt tillverkaren fortfarande sob 0,12. Aumlven om vaumlteperoxidhalten aumlr laringg kan den houmlga koncentrationen av oumlvriga kemikalier paringverka moumljligheten att faring fram DNA negativt Definição enligt Nationalencyklopedin Fluorescens, enform av utsaumlndande av ljus fraringn ett system. Fluorescens foumlrutsaumltter att systemet foumlrst har tillfoumlrts energi genom absorption av ljusstraringlning 12 Informationen i stycket aumlr haumlmtat fraringn Poppen M, 20022003, sid Informationen om Lumiscene aumlr haumlmtad fraringn 3 8 Vidare ger foumlrekomsten av fluorescein i loumlsningen moumljlighet till en extra kvalitetskontroll. Man kan bedoumlma om hela ytan har sprayats med loumlsningen genom att i efterhand kontrollera att den flourescerar vid belysning med blaringtt ljus. En extern ljuskaumllla och filter kan anvaumlndas foumlr att se var homem aplicador preparatet atrevido Lumiscene i sig fluorescerar naumlr det belyses med ljus mellan nm. Por favor, coloque-o no topo da página e deixe-o em conta. 3 Material och metoder 3.1 Lumiscenes kaumlnslighet foumlr blod jaumlmfoumlrt med Bluestar Prepararens kaumlnslighet foumlr blod undersoumlktes visuellt genom att goumlra en jaumlmfoumlrelse mellan kemiluminiscensen med Lumiscene och kemiluminiscensen med Bluestar. Nedan foumlljer en utfoumlrlig beskrivning foumlr hur testerna utfoumlrdes Anvaumlnda preparat Bluestar Forensic tablets 15. No seu melhor desempenho, você pode usar o Tablet Table de Som. Você está aqui: 125 ml destillerat vatten. Kit Lumiscene 16. Kit de armazenamento e armazenamento de 500 ml. Preparação Humal blod tappades i EDTA-roumlr. Blodet spaumlddes med vatten i olika koncentrationer 1:10, 1: 100, 1: 1000, 1:10 000, 1. 1: samt 1: Koncentrationerna valdes foumlr att faring en jaumlmn springning mellan alugar blod och mycket smaring koncentrationer. Jag ansaringg att det inte var var. Mening att garing laumlgre aumln 1: atrevido preparando-me e faço-me para criar um bom gosto em maumlngder blod det kan indikera. EDTA-roumlren med blod foumlrvarades fram till spaumldningen i kylskaringp. Spaumldningen utfoumlrdes i ett laboratorium paring Raumlttsmedicinalverket i Goumlteborg. Até o momento, é necessário aproveitar o programa de insumos, equipamentos e equipamentos, além de embalagens de plástico, 15 ml de plastificação 17. Blodloumlsningen droppades paring tvaring olika typer av provunderlag, oporoumlsa kakelplattor och poroumlsa filterpapper. Kakelplattorna var slaumlta (matta och graringfaumlrgade) utan moumlnster. Varje kakelplatta var 10x10 cm. Filterpappret var 15 Bestaumlllt genom CHASSE ET GRAPHIQUE HB, BLUESTAR Escandinávia. Generalagent och distributoumlr i Skandinavien, Island och Estland foumlr BLUESTAR. 16 Bestaumlllt genom Loci Forensic Products, Nieuw-Vennup, Holanda. 17 Centrifugroumlr pp steril, Fiscer Scientific, Goumlteborg, Sverige. 4 9 av maumlrket Munktell 18 och hade en diameter paring 9 cm. Foumlr att droppa blodloumlsningarna paring underlagen anvaumlndes en autopipett foumlr 200 l med engaringngsplastspetsar. Det placerades en droppe blodloumlsning por kakelplatta och por filterpapper. Det utfoumlrdes tre testomgaringngar. Infoumlr varje testomgaringng och foumlr varje blodloumlsningskoncentration preparerades tvaring filterpapper och tvaring kakelplattor, se figur 2 och tabell 1 foumlr uppstaumlllning. Det preparerades aumlven referensunderlag foumlr alla exponeringar i alla testomgaringngar. Referensunderlagen bestod av kakelplattor och filterpapper med blodloumlsningskoncentrationen 1: 100. Figur 2 Preparerade filterpapper och kakelplattor foumlr en testomgaringng Utfoumlrande Tvaring preparerade provunderlag placerades intill varandra foumlr att jaumlmfoumlras sida vid sida. Mellan underlagen placerades ett 40 cm houmlgt staumlnkskydd. Vid varje exponering sprayades ett provunderlag med Lumiscene och ett provunderlag med Bluestar. Med i varje exponering lades ett referensunderlag, i samma material som provunderlagen, med blodloumlsningen 1: 100, och detta referensunderlag pulverizações varje garingng med Lumiscene. Sob explosões, as pulverizações estão abaixo de 5 sekunder 5 sekunder. Utfoumlrandet upprepades foumlr varje blodloumlsningskoncentration. Figur 3 até Vulnerando a sua posição em frente e à frente de varandra, strecket i mitten visar staumlnkskaumlrmens placering. Até que você esteja satisfeito com essa experiência até ficar subentendida. 18 Munktell, Falun, Sverige. 5 10 Vid foumlrsta foumlrsoumlksomgaringngen pulverizações Bluestar med aerosolflaskan Ecospray 19 och Lumiscene med tillverkarens egen foumlr aumlndamaringlet avsedda pumpflaska ABA-spray. Flaskorna avger en vaumlsentlig skillnad i maumlngd vaumltska per tidsenhet, vilket bland annat kan ses paring underlagen efter sprayning 20. Tillverkaren av Lumiscene foumlrordar att pumpsprayflaskan eller liknande flaska som avger fin dimma anvaumlnds, daumlrfoumlr valdes aumlven att anvaumlnda sprayflaskan Ecospray vid en foumlrsoumlksomgaringng. Vid den andra foumlrsoumlksomgaringngen anvaumlndes saringledes aerosolflaskan até Baringde Bluestar och Lumiscene. Vid en tredje foumlrsoumlksomgaringng anvaumlndes pumpflaskan foumlr baringde Bluestar och Lumiscene. Figur 4 Anvaumlnda flaskor, Eco-spray até vaumlnster och ABA-spray até houmlger. Tabell 1 Oumlversikt av de tre testomgaringngarna. I varje testomgaringng och foumlr varje blodloumlsningskoncentration preparerades tvaring filterpapper och tvaring kakelplattor. Siffrorna i tabellen raiva spaumldningsfaktorn foumlr varje spaumldning av blodet. 1 Bluestar (Eco-spray) Lumiscene (ABA-spray) 1:10 1: 100 1: 1000 1. Bluestar (Eco-spray) Lumiscene (Eco-spray) 1:10 1: 100 1: 1000 1. Bluestar (ABA - spray) Lumiscene (ABA-spray) 1:10 1: 100 1: 1000 1. Eco-spray (Pulverizador ecológico), Labo Chimie França, Meyreuil, França. 20 Bild paring provunderlagens utseende efter sprayning, se bilaga 1 6 11 3.2 Specificitet Foumlr att undersoumlka Lumiscenos specificitet applicerades paring kakelplattor besudlingar fraringn aumlmnen som kunde taumlnkas ge falska positiva reaktioner. Kakelplattor foumlr tvaring testomgaringngar preparerades och utfoumlrdes med 12 veckors mellanrum. Aumlmnena valdes ut efter vad tidigare undersoumlkningar visat angaringende falska positiva reaktioner foumlr blodvisualiseringsmedel som luminol, Bluestar och fluorescein 21 homens aumlven foumlr vissa blodfoumlrproumlvningsmedel, som t ex Leukomalakitgroumlnt 22. Tabell 2 Oumlversikt paring de aumlmnen som valdes ut foumlr test av falska positiva reaktioner. Uppstaumlllningen av aumlmnena i tabellen aumlr densamma som deras placering paring kakelplattorna foumlr respektive testomgaringng. Jodopax Motorolja Pepparot Blod 1: 100 C-vitamina Potatis Raumldisa KlorRent Jaumlrntillskott Roumldloumlk Blod 1: 100 Cillit Bang Karinglrot Palsternacka Virkon Sisal Jordgubbar Blod 1: 100 Djurblod (gris) Hampa Granataumlpple Hallon Testomgaringng 1 Testomgaringng 2 Figur 5 Prepararade kakelplattor foumlr testomgaringng 1 och Son Bu-Nam, Validação do novo kit de detecção de mancha de sangue latente Hemascein, Gyeonnam Korea. Na apresentação do Powerpoint, compara-se o cd-skiva levererad Av Abacus Diagnostics, West Hills CA, EUA. 22 Groumlnlund C, 20082009, sid 12. 7 12 3.3 Fotodokumentation Dokumentation av reaktionerna, avseende baringde kaumlnslighet och specificitet, skedde genom fotografering. Até fotograferingen anvaumlndes kameran Nikon D80 paring stativ med reproarm, daumlr kameran placerades vinkelraumlt mot underlaget. Exponeringsinstaumlllningen valdes até slutartiden 30 sekunder, blaumlndartal 8 och ISO 800. Exponeringsinstaumlllningarna valdes efter samtal med fotograf Marcus Andrae samt efter ett antal provomgaringngar i det aktuella undersoumlkningsutrymmet. 3.4 DNA-analys Foumlrsoumlken straumlvade efter att undersoumlka Lumiscenes eventuella paringverkan paring efterfoumlljande DNA-analisador. Filterpapper preparerades med blodloumlsningar foumlr sex testomgaringngar. En testomgaringng innebar ett filterpapper por blodloumlsningskoncentration. Instrutores de produtos de confecção de lenços e lâminas de sopro de lâmpadas e lâminas de ferragens. Paring tvaring testomgaringngar, som utgjorde referensmaterialet, applicersades varken Lumiscene eller Bluestar. Referensmaterialet bestod alltsaring endast av blodloumlsningarna paring filterpapper. Aplicando-se a partir de Lumiscene och Bluestar com o método de pulverização med Eco-sprayflaska sekunder. Det saumlkrade materialet fraringn varje testomgaringng, totalt 28 filterpapper, skickades até biologienheten paring Statens kriminaltekniska laboratorium daumlr de undersoumlktes med standardmetoden 23. Até o foumlrsoumlken valdes filterpappren framfoumlr kakelplattorna eftersom blodbesudlingarna paring de oporoumlsa kakelplattorna hade en foumlrmaringga att loumlsas upp och spridas ut. En felkaumllla skulle ousado kunna bli att man med tops inte faringr med hela blodbesudlingen. 3.5 Avgraumlnsningar Undersoumlkningen har skett paring tvaring typer av underlag, poroumlst och oporoumlst. Av tidsekonomiska skaumll har arbetet begraumlnsats até endast ett poroumlst underlag (filterpapper) och ett oporoumlst underlag (kakelplattor). Eu arbetets boumlrjan fanns ambitionen att jaumlmfoumlra Lumiscene med det fluoresceinbaserade preparatet Hemascein 24 avseende kaumlnslighet foumlr blod. Por favor, entre em contato com o usuário, por favor, envie-me para o seu site e forneça-lhe um email para a empresa. Hemascein slutar ge fluorescens......................................................................................... Jag bedoumlmde dock inte denna jaumlmfoumlrelse som tillraumlckligt relevante att utfoumlras p g a tidsekonomiska skaumll. 23 B-SF03 Standardfoumlrfarande foumlr DNA-analys hos Statens kriminaltekniska laboratorium. 24 Hemascein aumlr ett utvecklat kit baserat paring fluoresceinmetoden, tillverkas av Abacus Diagnostics, West Hills CA, EUA. 8 13 3.6 Felkaumlllor Samtliga foumlrsoumlksomgaringngar efterstraumlvades att haringllas konstanta, utan ooumlnskade variationer. Os homens que se atrevem a se tornar um animal de estimação podem ser tratados com uma variação. En variation som fraumlmst skulle kunna paringverka resultatet aumlr hur stor maumlngd av preparaten som aplicadores paring underlagen. Preparar as pulverizações que se partem abaixo da superfície da água e do solo sob a forma de uma laringning tid foumlr alla foumlrsoumlkomgaringngar, homens aringterigen, ousado detta skett manuellt kan mindre variationer ha foumlrekommit. 4 Resultat 4.1 Kaumlnslighet foumlr blod Oberoende om Lumiscene sprayas med aerosolflaska eller pumpflaska blir reaktionen med Lumiscene starkare aumln den med Bluestar. I de houmlgre blodloumlsningskoncentrationerna 1:10, 1: 100 och 1: 1000 kan reaktionen efter applicering med Lumiscene med laumltthet ses paring flera metros avstaringnd medan reaktionen efter aplicando med Bluestar ses allra baumlst paring naumlra avstaringnd. Reaktionen efter applicering av Lumiscene aumlr synlig naringgra sekunder laumlngre aumln reaktionen som blir efter applicering av Bluestar. O que é o que é o que é o que você quer dizer é que você pode usar o Lumiscene som applicerades. Foumlr de houmlgre blodloumlsningskoncentrationerna gavs en reaktion med Lumiscene som varade upp till flera minuter efter sprayning. Aparas filterpapper gav Lumiscene en synlig reacional ner até blodloumlsningskoncentrationen 1. Bluestar gav en ner synlig reacional até blodloumlsningskoncentrationen 1: Aparas kakelplattor GAV Lumiscene en mycket svag reacional aumlnda ner até blodloumlsningskoncentrationen 1. Bluestar gav en ner synlig reacional até blodloumlsningskoncentrationen 1: Intensiteten paring kemiluminiscensen jaumlmfoumlrdes Ockulaumlrt. Jaumlmfoumlrelsen gjordes i huvudsak efteraringt genom att studera kemiluminiscensen paring fotodokumentationen. En viss jaumlmfoumlrelse gjordes aumlven direkt vid sprayeneden ouing det var omoumljligt att inte bilda sig en uppfattning om intensiteten paring kemiluminiscensen ousado den betraktades direkt. Dokumentationen fraringn de olika sprayningarna aumlr uppstaumlllda i sex olika grupper med foton. Oumlverst i varje grupp med foton finns angivet de foumlrutsaumlttningar som gaumlller foumlr respektive grupp, allt vilka sprayflaskor och vilket underlag som anvaumlnts. Nedan redovisas tvaring grupper med foton en grupp foumlr pulverização med anvaumlndning av ABA-sprayflaskan paring underlaget filterpapper (Fig. 6) och en grupp foumlr pulverização med anvaumlndning av ABAsprayflaskan paring kakelplattor (figur 7). Oumlvriga foton redovisas i bilaga 2. 9 14 Bluestar (ABA-spray) - Lumiscene (ABA-spray) Filterpapper BLBL Ref 1: 100 Ref 1: 100 Blodloumlsning 1: 10 Blodloumlsning 1: 100 BLBL Ref 1: 100 Ref 1: 100 Blodloumlsning 1: 1000 Blodloumlsning 1: BLBL Ref 1: 100 Ref 1: 100 Blodloumlsning 1: Blodloumlsning 1: BL Ref 1: 100 Blodloumlsning 1: Figur 6 Foumlr varje bild gaumlller: Underlaget até vaumlnster har sprayats med Bluestar (B) och underlagen till houmlger Haras de spray med Lumiscene (L). Underlaget laumlngster ner till houmlger aumlr referensunderlag med blodloumlsning 1: 15 Bluestar (ABA-spray) - Lumiscene (ABA-spray) Kakelplattor BLBL Ref 1: 100 Ref 1: 100 Blodloumlsning 1:10 Blodloumlsning 1: 100 BLBL Ref 1: 100 Ref 1 : 100 Blodloumlsning 1: 1000 Blodloumlsning 1: BLBL Ref 1: 100 Ref 1: 100 Blodloumlsning 1: Blodloumlsning 1: BL Ref 1: 100 Blodloumlsning 1: Figur 7 Foumlr varje bild gaumlller: Underlaget até vaumlnster har sprayats med Bluestar (B) och Underlagen até houmlger har sprayats med Lumiscene (L). Underlaget laumlngster ner till houmlger aumlr referensunderlag med blodloumlsning 1: 16 4.2 Specificitet Av de aumlmnen som undersoumlktes gav djurblod, karinglrot, raumldisa, pepparrot, Virkon och KlorRent positiva reaktioner i den foumlrsta testomgaringngen. Med positiv reaktion menas haumlr en synlig kemiluminiscens. Rengoumlringsmedlet Virkon gav visserligen em positiv reaktion men den var naringgot svagare aumln de oumlvriga. Rengoumlringsmedlet KlorRent gav em stark positiv reaktion, homens reaktionen skilde sig markant fraringn den reaktion som uppkom foumlr de oumlvriga aumlmnena och aumlven foumlr den som uppkommer foumlr humant blod. Reaktionen foumlr KlorRent ser ut att vara roumlrlig paring saring saumltt att det ser ut att brinna eller ryka. De preferência, reaktionerna ses som ett blaringtt ljus i figur 8 och figur 9. Foumlr de olika aumlmnenas placering paring kakelplattorna se tabell 2. Figur 8 Kakelplattor efter sprayning med Lumiscene i testomgaringng 1. Till houmlger ses tre referensdroppar med blodloumlsningen 1: 100. Efter 12 veckor var det endast djurblod och referensblodloumlsningarna som gav positiva reaktioner. 12 17 Figur 9 Kakelplattor efter sprayning med Lumiscene i testomgaringng 2. Till houmlger ses tre referensdroppar med blodloumlsningen 1: análise de DNA analisador de DNA genomfoumlrdes enligt metodbeskrivningen sob rubrik 3.4. Inga DNA-profiler erhoumllls dock i testerna, varken paring referensmaterialet eller paring proverna som applicers at Lumiscene eller Bluestar. DNAextraktionen misslyckades pga att filterpapperen loumlste upp sig i roumlren och blev till en soumlrja. 4.4 Anvaumlndarvaumlnlighet Produkterna som anvaumlndes i foumlrsoumlken var faumlrdiga kit som endast behoumlvde ett eller ett par handhavanden foumlr att resulttera i en arbetsloumlsning faumlrdig att anvaumlnda. Lumiscenes anvaumlndarvaumlnlighet i faumlltbruk var lika tillfredsstaumlllande som Bluestars. Lumiscene aumlr enkel att anvaumlnda, tar kort tid att foumlrbereda och risken foumlr felhantering aumlr mínimo. Bluestar kan dock bestaumlllas som comprimido daumlr man blandar ut med destillerat vatten eller som grundloumlsning med aktiveringstabletter. Detends flera valmoumljligheter foumlr att kunna bestaumllla mindre kit, alltsaring mindre maumlngd vaumltska per omgaringng. Lumiscene kan i dagslaumlget endast bestaumlllas i kit av en storlek. Maumlngden faumlrdig arbetsloumlsning som erharinglls raumlcker gott och vaumll até en normalstor laumlgenhet, oanvaumlnd maumlngd vaumltska faringr kastas. Inkoumlpspriset foumlr Bluestar aumlr dock houmlgre vilket goumlr att Lumiscene aumlndaring blir billigare att anvaumlnda. 13 18 Tabell 3 Kostnad Kostnad por ml Kostnad por kit Lumiscene LUM kr 30 EUR fraktkostnad 24 EUR total kostnad 54 EUR 499 kr Forex saumlljkurs (ger 500 ml) Bluestar Bluestar comprimidos Forensic Koumlpt fraringn Loci Forensic Products 1,55 kr 775 kr foumlr 4 doseador (ger 500 ml) Koumlpt fraringn Christer Holmgren Bluestar Escandinávia Tillverkaren av Lumiscene ira att naumlr vaumll aktiveringstabletterna blandats med grundloumlsningen ska den faumlrdiga arbetsloumlsningen vara stabil och anvaumlndbar 4-6 timmar i rumstemperatur. Vid sidan av de oumlvriga praktiska foumlrsoumlken foumlrvarades en flaska med faumlrdigblandad arbetsloumlsning Lumiscene ca 4 veckor i kylskaringp, innan det understmlktes om den fortfarande fungerade. Pulverizações Lumiscene paring brunpapper daumlr det preparerats med blodloumlsning av djurblod i koncentrationen 1:10. Resultatet blev en klart synlig reaktion, aumlven om den var svagare aumln vid de oumlvriga foumlrsoumlken ousar arbetsloumlsningen var faumlrsk. Om en aktiverad arbetsloumlsning med Lumiscene foumlrvaras i kylskaringp garingr den att anvaumlnda aumlven efter de timmar som rekommenderas, homem homem boumlr ta i beaktande att kaumlnsligheten avtar med tiden. 14 19 5 Diskussion 5.1 Kaumlnslighet foumlr blod Foumlrsoumlken visade att Lumiscene gav en starkare reaktionen foumlr alla blodkoncentrationer. Tillverkaren, Loci Forensic Products, uppger som tidigare naumlmnts att reaktionen fraringn Lumiscene avger ljus med varingglaumlngder kring 525 nm och att omlgats celler aumlr som kaumlnsligast foumlr ljus med just denna varingglaumlngd. Fraringgan aumlr om Lumiscene som preparat aumlr kaumlnsligare foumlr blod eller u det upplevs som saring tack vare att ljuset fraringn Lumiscene aumlr laumlttare att uppfatta. Oavsett orsak saring aumlr resultatet att Lumiscene aumlr mer laumlttarbetat tack vare den starkare reaktionen. Risken att missa latente blod borde daumlrfoumlr bli mindre. Den starkare reaktionen bidrog aumlven até att mindre moumlrker kraumlvdes foumlr att se reaktionen. Eftersom reaktionen varade flera sekunder laumlngre med Lumiscene underlaumlttades fotograferingen. Paring en brottsplats audaz det ofta aumlr flera undersoumlkare naumlrvarande skulle detta dessutom moumljliggoumlra en laumlngre diskussion mellan undersoumlkarna om det visualiserade blodets placering och reaktionens utseende. Naumlr metoderna foumlr visualisering av latent blod blir baumlttre och baumlttre uppstaringr fraringgan hur smaring blodmaumlngder det aumlr intressanta att kunna paringvisa i ett kriminaltekniskt sammanhang och om det finns naringgra risker med att metoderna blir foumlr kaumlnsliga. Efter att man erharingllit en kemiluminiscens, en indikation paring blod, med hjaumllp av en blodvisualiseringsmetod vom man kanske med ett blodfoumlrproumlvningsmedel ytterligare kontrollera att det verkligen aumlr blod. Det skulle ousado kunna uppstaring ett informationstomrum om blodvisualiseringsmetoderna aumlr mycket kaumlnsligare foumlr blod aumln vad blodfoumlrproumlvningsmedlen aumlr. Personligen anser jag dock att en foumlrproumlvning med ett blodfoumlrproumlvningsmedel efter att ha erharingllit en kemiluminiscens inte i tillraumlcklig maringn staumlrker paringstaringendet att det verkligen aumlr blod, detta paring grund av att felkaumlllorna foumlr blodfoumlrproumlvningsmedlen aumlr snarlika dem foumlr blodvisualiseringsmetoderna. Jag anser daumlremot att det aumlr oumlnskvaumlrt att blodvisualiseringsmetoderna aumlr ungefaumlr lika kaumlnsliga som metoderna foumlr att paringvisa och bestaumlmma DNA. I vissa aumlrenden finns det naturligtvis en vinst i att endast kunna visualisera blod men i de allra flesta aumlrenden aumlr det oumlnskvaumlrt att aumlven kunna saumlga vems blod det aumlr. 5.2 Specificitet De falska positiva reaktionerna gavs av ungefaumlr samma aumlmnen som tidigare studier visat foumlr luminol, Bluestar och fluorescein. Foumlrsoumlken visade inte oumlverdrivet maringnga falska positiva reaktioner men heller inte faumlrre aumln foumlr de oumlvriga preparaten. Utseendet paring reaktionen foumlr KlorRent var vaumlntad eftersom den reaktionen aumlr typisk aumlven foumlr Bluestar. Den annorlunda reaktionen goumlr det moumljligt att saumlrskilja en reaktion foumlr blod och en reaktion 15 20 foumlr klorin. Naumlr det gaumlller specificiteten aumlr Lumiscene varken baumlttre eller saumlmre aumln sina konkurrenter. Foumlrsoumlken fraringn den andra testomgaringngen visade att klorin och andra aumlmnen som gav falska positiva reaktioner i foumlrsta testomgaringngen inte gjorde det i andra testomgaringngen. Humant blod och djurblod gav fortfarande positiv reaktion. Det kan betyda att risken foumlr att faring falska positiva reaktioner av t ex klorin paring en tvaumlttad brottsplats minskar med tiden. 5.3 DNA-analys DNA-analyserna misslyckades pga att filterpapperen loumlste upp sig i roumlren och blev till en soumlrja. Eftersom testerna angaringende Lumiscenes paringverkan paring DNA misslyckades garingr det inte att dra naringgra slutsatser. Teoretiskt kan man saumlga att Lumiscene foumlrvaumlntas paringverka DNA-analyserna mindre negativt aumln Bluestar och de klassiska luminolloumlsningarna som Grodksy - och Weberloumlsningar 25, eftersom Lumiscene inneharingller mindre vaumlteperoxid. Filterpapprens egenskaper gjorde dem olaumlmpliga foumlr anvaumlndning vid DNAanalyser. Jag rekommenderar att inte anvaumlnda filterpapper till blod eller sekret som sedan skall DNA-undersoumlkas. 5.4 Anvaumlndarvaumlnlighet Lumiscene anvaumlnds i stort sett paring samma saumltt som Bluestar. Det Bluestar-kit jag anvaumlnde i undersoumlkningen kraumlvde tillgaringng till destillerat vatten som aktiveringstabletterna skall loumlsas upp i medan Lumiscene-kitet redan innehoumlll allt som kraumlvdes. I oumlvrigt aumlr baringda preparaten mycket enkla att anvaumlnda paring en brottsplats, de har samma korta foumlrberedelsetid och de kraumlver inte stor arbetsyta foumlr att foumlrbereda. Eftersom kemiluminiscensen fraringn Lumiscene aumlr stark garingr den att se med blotta oumlgat aumlven om undersoumlkningsrummet inte aumlr totalt moumlrklagt. Detta kan underlaumltta paring brottsplatser daumlr foumlrutsaumlttningarna varierar och det i vissa fall inte aumlr moumljligt att faring helt moumlrklagt. Ur kostnadssynpunkt finns det inget hinder att boumlrja anvaumlnda Lumiscene vid flera tekniska rotlar. Det visade sig vara billigare att koumlpa in Lumiscene aumln Bluestar. Vid testerna anvaumlndes baringde ABA-sprayflaskan och Eco-sprayflaskan. Ecosprayflaskan ger en finare dimma vilket aumlr att foumlredra eftersom en fin dimma ger en baumlttre spridning utan att den lilla blodmaumlngden man soumlker efter rinner undan. Med ABA-sprayflaskan maringste man pumpa upp ett tryck med jaumlmna 25 Grodsky utvecklade en luminolloumlsning 1951 som i grunden fortfarande anvaumlnds i kriminaltekniska sammanhang. Weber vidareutvecklade metoden Kaumllla 16 21 mellanrum. Med Eco-sprayflaskan faringr man en kontinuitet i appliceringen eftersom det garingr att spraya oavbrutet tills gasen aumlr slut. 5.5 Foumlrslag till fortsatta undersoumlkningar Arbetsmiljouml och haumllsa Arbetet har inte beroumlrt fraringgan om naringgon av metoderna som anvaumlnds foumlr att visualisera latent blod aumlr farliga foumlr haumllsan och om det i det avseendet skiljer sig naringgot mellan preparaten. Jag taumlnker daring fraumlmst paring haumllsan fraringn en brottsplatsundersoumlkares perspektiv. Metoden i sig kraumlver att vi anvaumlnder en sprayflaska som ger en fin dimma, en fin dimma som vi andas in om vi inte anvaumlnder naringgon typ av andningsskydd. Vid bestaumlllning av preparaten naumlmner tillverkarna inget om att andningsskydd skall anvaumlndas och i saring fall vilket andningsskydd som aumlr laumlmpligt. Men det aumlr till sist och synes inte tillverkaren som har stoumlrsta ansvaret foumlr en brottsplatsunderoumlkares haumllsa utan det har brottsplatsundersoumlkaren sjaumllv. Jag rekommenderar att andningsskydd anvaumlnds eftersom vaumlteperoxid aumlr ett starkt oxidationsmedel. Dessutom aumlr luminols skadliga effekter paring maumlnniskans haumllsa aumlnnu inte helt kartlagda. Det aumlr daumlrfoumlr viktigt att skydda sig fraringn inandning och onoumldig hudkontakt. Mitt foumlrslag paring en fortsatt undersoumlkning aumlr att belysa detta eftersatta men viktiga omraringde Tid - och temperaturparingverkan paring preparates haringllbarhet Tillverkaren Loci Forensic Products rekommenderar att den faumlrdiga arbetsloumlsningen Lumiscene anvaumlnds inom 4 timmar. Daumlrfoumlr kan det vara av intresse att undersoumlka hur foumlrvaring under olika tider och olika temperaturer paringverkar loumlsningens kaumlnslighet foumlr blod Paringverkan paring efterfoumlljande foumlrproumlvningsmetoder Kontrollera hur upprepade sprayningar med luminol, Bluestar och lumiscene paringverkar kaumlnsligheten hos efterfoumlljande test med antikroppsbaserade humanspecifika foumlrproumlvningsmetoder foumlr blod. Luminol appliceras i en basisk loumlsning 26 vilket kan paringverka antikropparna i dessa tester. 26 Definition enligt Nationalencyklopedin en loumlsning som har ett PH-vaumlrde oumlver 7. 17 22 5.6 Slutsats Reaktionen med Lumiscene emitterar starkare ljus aumln reaktionen med Bluestar. Lumiscene upplevs kaumlnsligare aumln Bluestar. Det garingr dock inte att saumlga om detta beror paring att den kemiska reaktionen sker effektivare med Lumiscene eller om det beror paring att det emitterade ljuset har varingglaumlngder kring 525 nm som oumlgat har laumlttare att uppfatta. Lumiscene aumlr varken baumlttre eller saumlmre aumln Bluestar naumlr det gaumlller specificitet foumlr blod. Naringgon slutsats angaringende paringverkan paring efterfoumlljande DNA-analyser garingr inte att goumlra paring grund av ofullstaumlndigt testresultat. En mindre risk foumlr nedbrytning av DNA boumlr dock foumlreligga med Lumiscene. Lumiscene aumlr lika anvaumlndarvaumlnlig som Bluestar. Lumiscene aumlr mer kostnadseffektivt aumln Bluestar. 18 23 6 Kaumlllfoumlrteckning 6.1 Litteratur Garcia-Campana Ana M et al, Chemiluminescens in Analythical Chemistry. Groumlnlund Carina, En studie i roumltt en jaumlmfoumlrande studie av sex blodfoumlrproumlvningsmedel. Specialarbete vid den kriminaltekniska grundutbildningen paring SKL. Poppen Marie, Fluorescein en metod foumlr att framkalla latent blod. Specialarbete vid den kriminaltekniska grundutbildningen paring SKL. Son Bu-Nam. Validation of novel latent bloodstain detection kit Hemascein. The 20th Conference of Korean Society of Forensic Science. En powerpoint presentation paring cd-skiva levererad 2011 av Abacus Diagnostics, West Hills CA, USA. 6.2 Muntliga kaumlllor Anders Nilsson, biologienheten paring Statens kriminaltekniska laboratorium, januari-september Christer Holmgren, CHASSE ET GRAPHIQUE HB, BLUESTAR Scandinavia. Generalagent och distributoumlr i Skandinavien, Island och Estland foumlr BLUESTAR, Martin Eversdijk, Loci Forensic Products, Rene Gelderman, Loci Forensic Products, januari-maj Marcus Andrae, fotograf paring Statens kriminaltekniska laboratorium, april Internet t om t om t om 24 7 Bilagor 7.1 Bilaga 1 Bild ovan visar underlag efter sprayning med Eco-sprayflaska 55 sek. Bild ovan visar underlag efter sprayning med ABA-sprayflaska 55 sek. 20 25 7.2 Bilaga 2 Bluestar (Eco-spray) Lumiscene (Eco-spray) Filterpapper Blodloumlsning 1:10 Blodloumlsning 1:100 Blodloumlsning 1:1000 Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Foumlr varje bild gaumlller: Underlaget till vaumlnster har sprayats med Bluestar (B) och underlagen till houmlger har sprayats med Lumiscene (L). Underlaget laumlngst ner till houmlger aumlr referensunderlag med blodloumlsning 1: 26 Bluestar (Eco-spray) Lumiscene (Eco-spray) Kakelplattor Blodloumlsning 1:10 Blodloumlsning 1:100 Blodloumlsning 1:1000 Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Foumlr varje bild gaumlller: Underlaget till vaumlnster har sprayats med Bluestar (B) och underlagen till houmlger har sprayats med Lumiscene (L). Underlaget laumlngst ner till houmlger aumlr referensunderlag med blodloumlsning 1: 27 Bluestar (Eco-spray) Lumiscene (ABA-spray) Filterpapper Blodloumlsning 1:10 Blodloumlsning 1:100 Blodloumlsning 1:1000 Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Foumlr varje bild gaumlller: Underlaget till vaumlnster har sprayats med Bluestar (B) och underlagen till houmlger har sprayats med Lumiscene (L). Underlaget laumlngst ner till houmlger aumlr referensunderlag med blodloumlsning 1: 28 Bluestar (Eco-spray)- Lumiscene (ABA-spray) Kakelplattor Blodloumlsning 1:10 Blodloumlsning 1:100 Blodloumlsning 1:1000 Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Blodloumlsning 1: Foumlr varje bild gaumlller: Underlaget till vaumlnster har sprayats med Bluestar (B) och underlagen till houmlger har sprayats med Lumiscene (L). Underlaget laumlngst ner till houmlger aumlr referensunderlag med blodloumlsning 1:HIQ. CELEBRATES ITS 20TH BIRTHDAY HiQ SIMPLIFIES EVERYDAY LIFE Its easy to talk about simplicity, the difficult thing is simplifying. Our industry has been living with this challenge for a long time. Simplicity is a keyword at HiQ, as we create services to improve and simplify peoples lives. Just think of how easy it is to submit your income tax return or transfer money using your smartphone. We believe that IT that simplifies equates with quality. Simplicity is the innovation of our time. We live in a world in which innovative IT-based services and products have improved the everyday lives of people. We see for example how social media help to bring about the downfall of dictatorships, how communication channels evolve and how very valuable IT has become for individuals, companies and organisations. We take on the challenges with enthusiasm and expertise. We listen to our clients. We are the specialists who can get to grips with the intricacies of technology and the clients requirements and there to propose the simple solution. As a result, we create services and products that improve and simplify peoples lives right in the midst of the adventure that we call IT. 1992 HiQ grew up out of the formation of the company Statyetten Konsult AB and its subsidiary, Approve AB. The focus of the groups operations was primarily simulation. 1995 HiQ was formed in Stockholm, with the task of expanding operations with a focus on telecommunications. 1998 The parent company was renamed HiQ International AB. 1998 Subsidiaries were formed in Gothenburg and Oslo. 1999 HiQ International was listed on the Stockholm Stock Exchange. 2000 Two new subsidiaries acquired, HiQ Wise in Denmark and HiQ Open in Gothenburg. 2000 Subsidiary established in Finland, HiQ East Oy. 2002 Softplan Oy acquired in Finland. 2002 New subsidiary, HiQ Skaringne AB, established in Lund. HiQ East Oy and HiQ AS were liquidated. 2003 HiQ Vaumlst and HiQ Open merge into one company, HiQ Goumlteborg. 2004 Computer and Audio-Technical Systems AB acquired. 2004 New subsidiary started, HiQ Karlskrona AB. 2007 MobilEyes AB acquired. 2008 Ace Simulation AB acquired. 2008 TSG Test Solutions acquired. HiQS OPERATIONS ARE CONDUCTED THROUGH OUR VARIOUS SUBSIDIARIES. We have offices in the Nordic region and Eastern Europe - from the Oumlresund Region in the south, to Stockholm and Helsinki in the north and to Moscow in the east. From our offices in Sweden, Finland, Denmark and Russia, we offer specialised services within communications, software development and business-critical IT. HiQ Stockholm is located in the heart of the Swedish capital and has some of the best views of the city available. With more than 400 employees, we create results and simplify for clients from other parts of the country, the region and around the world. We help customers in segments such as telecom, defence and security, finance and insurance, industry and retail, gaming, entertainment and media and the public sector. We offer everything from strategy consulting, business development and software development to testing. Our customers are not looking primarily for our resources and a few extra hands, but for our minds and innovative abilities. Although we work across many industries and supply a wide range of services, we are specialists with an in-depth understanding of the fields and domains in which we work. We understand the need to place technology within a context and how technology affects the people who work with it and our customers business in general. Simplicity is difficult to achieve, but for us it is a necessary way of approaching assignments to ensure we deliver useful and efficient solutions to difficult problems. It takes courage to step away from conventional thinking in order to seek better solutions to our customers challenges. Employees at HiQ Stockholm are daring and creative team players. Could you be one of us HiQ Goumlteborg has been active in the heart of the technology-intensive Gothenburg region since 1998. HiQ Gothenburg has over 300 specialists with expertise in fields such as software development (from start to finished product), quality management (especially testing and verification), qualified project management and business development, mobility and web development. We are very flexible when it comes to forms of cooperation with our customers - we can work either at your location or at our own offices. We can participate in our customers projects, or run an entire project either at your premises or at our offices. Approximately two-thirds of our business is conducted on-site with our customers and the remainder at our offices, often in the form of project assignments. We have successfully carried out numerous projects at our offices and have extensive experience, expertise and structural investment in this form of working. We are located in Arboga, Borlaumlnge and Vaumlsterarings and work with advanced IT development throughout the western Maumllardalen region. HiQ Maumllardalen is one of the largest IT consulting company in the western Maumllardalen region, with about 100 consultants. We work with systems development and project management in both product development and operations and we have successfully conducted complex development in our own premises for over 16 years. We are also a leader in quality assurance. We work both on site at the customer and with larger project commitments in our own premises. We are operating in the region around Oumlrebro-Vaumlsterarings-Eskilstuna and in Borlange-Ludvika-Falun and some of our world leading clients are ABB, Atlas Copco, BAE Systems Bofors Haumlgglund, Bombardier, FMV, FM, Partex, SEB, Transportation Board, Volvo and Westinghouse. Today we see a strong demand for our services and are therefore on the look out for new talented consultants who want to work with systems development or project management in the IT field, preferably with experience from one or more of segments the automotive, defense or industry On the Swedish side of the technology and innovation-intensive Oumlresund Region, HiQ Skaringne has been active since 2002. We are a stable and expanding service company with about a hundred employees and have established ourselves as a leading partner both for highly specialised and innovative companies and for global industries. Successful turnkey projects completed for our local customers have allowed us to establish ourselves as an international player with clients in commerce, telecom, electronics, media, finance and medicin in both the European continent and in North America. Our expertise extends from product development (e. g. embedded system for controlling electronics) to business support (complete systems for e-commerce). Accepting full responsibility for projects is just as common for us as providing expert consultants to work on-site with our clients. At our offices in Lund, we have centres for mobile application development, web services development and quality assurance. At HiQ Karlskrona we have extensive know-how and experts on software development, quality assurance and leadership. The company was established in 2004 when HiQ began operations at its own premises in Blekinge. The region is home to some of HiQs most important contracted customers, like Telenor and Ericsson. We work closely with our customers, developing their business, technology and operations. Some of our areas of expertise are: IT and Business Solutions. We offer specialist competence within IT management, project management, system development, quality assurance, testing, web solutions, strategies and digital communication. Mobile Solutions. The mobile trend has only just started and mobile devices and platforms are taking an increased role in our everyday life as well as in companies and organizations. At HiQ Karlskrona we offer mobile solutions to streamline your business and meet the needs of your customers. Please contact us and let us tell you more over a cup of coffee HiQ Ace Linkoumlping HiQ Ace is located in Swedens bustling, fourth-largest metropolitan region in Oumlstergoumltland County. We have provided software development expertise for the automotive, defence and telecom industries for a number of years, working together with world-leading clients from the region. HiQ Ace is now adding business-critical IT to our offering and helping our customers develop processes and IT support. HiQ Ace is an entrepreneur-driven company that is growing fast. Our consultants are skilled at solving the most difficult of technical problems. On the other hand, its in our nature to try not to complicate things unnecessarily, but to help each other and our customers to see what possibilities are available. Oh, and having fun is a fringe benefit when you work with HiQ HiQs Russian office is located in central Moscow and has been running successful nearshore projects since 1999. We specialise in all open mobile platforms, finance, gaming, entertainment and media. Our colleagues in Moscow have many years of experience handling development work for international clients and, needless to say, all our staff here speak English. We possess the same high level of quality awareness here at our nearshore centre as at all our other HiQ offices. With our nearshore model, HiQ offers cost-effective solutions without compromising quality, budget or deadlines. HiQs model offers a flexible solution in that we can assume full responsibility for projects with the help of a project coordinator from one of our local offices. We like to work using the agile Scrum method, but, naturally, are also happy to adapt and use whatever methods our customers prefer. This is outsourcing 2.0 Located in Espoo, in the heart of Finlands high-tech area just outside Helsinki, HiQ Finland specialises in challenging systems development projects, quality assurance, project management and consultancy. The company is one of the oldest within the Finnish IT sector and consists of a highly experienced team of experts. Our core expertise lies in areas such as e-commerce solutions using mobile and web-based portals, business-critical systems, interactive gaming platforms, telecommunications systems, network services within telecom, and mobile applications. With our unique understanding of business process integration and management, we help our customers get maximum value out of their existing IT environments. We have created innovative systems and solutions for a number of Finnish and international organisations. HiQ Finland also holds a strong position within quality assurance - with a complete offering that covers the entire process from strategy, methodology and management to implementation. Rock star amp entrepreneur Today, digital solutions are a business critical part in all industries, and the music industry has received great attention for the challenges in finding new ways to distribute music and earn money. HiQ works with many companies in this area and is also digital partner to Dregen - Swedens biggest rock star. HiQ and Dregen have collaborated for several years and as a digital partner HiQ works with developing Dregens digital platforms to help him reach out to his fans. In the beginning of 2014 Dregen launched his new modern website. created by HiQ. Dregen is also a familiar face at HiQs many events, such as our rock school and the Knowledge Bar, where he spreads energy and inspiration and plays with the HiQ band HEO. When the music video for Just like that was to be made, Dregen chose HiQs offices as the location - below is a video behind the scenes of the shoot HiQs own motor sports star. As a specialised IT consultancy firm, HiQ works with leading customers in the automotive industry in areas such as Infotainment and Active Safety. Motorsport is a technology-intensive team sport, with IT increasingly playing a part in performance, safety and contacts with fans and the media. Richard Goumlranssons career started at the age of ten with go-cart and through hard work he has since established himself in the elite of racing and rally. HiQ supports Richard both on the race track and by together creating thrilling activities to share with our clients and employees. The Maths Centre Maths is the first step. At HiQ we believe that it is important that young men and women discover and learn to understand mathematics at an early age. Both for their own sake as well as to secure competence for the future. Therefore, we work with the non-profit organisation Mattecentrum (The Maths Centre). At no charge Mattecentrum helps over 120 000 children and youths to improve their skills in maths. This is done partly through open workshops, where HiQ employees are among the volunteers, but foremost by the online study tool matteboken. se (matteboken is the Swedish word for The maths book). Matteboken offers theory, video lessons, and excercises, with complete maths books from the Swedish A-E maths courses. Matteboken also contains a forum where users can ask questions. Who knows, one of the thousands of young people getting help from Mattecentrum might work for HiQ one day HiQ Sailing Team 2002-2008 HiQ had a very successful collaboration with the sailing project HiQ Sailing Team. HiQ Sailing Team, the well-known 60-foot trimaran and one of the fastest sailing boats in the world, has broken a number of records through the years. Among the most spectacular were the events of December 2006, when HiQ Sailing Team sailed around Gotland in a new record time of 17 hours 39 minutes - at an average speed of nearly 21 knots The trimaran has given us amazing experiences with both customers and staff, a great deal of pride and attention. It has been a true journey writing record history together. European Athletics indoor championships Non-Hit Car amp Truck - simpler, safer driving Challenge While driving a car you are constantly exposed to hazards in the form of, for example, other road users and wild animals. Remaining alert to all of these hazards unaided is not always an easy task. Assignment Helping the client Volvo Cars to make use of advanced technology to create solutions that mean that the vehicle itself is able to detect the hazards that can occur in traffic and on the road. These solutions are to contribute to the vision that by the year 2020 there will be no fatalities or serious injuries to anyone travelling in a Volvo car. Solution As part of the four-year Non-Hit Car project, HiQ is playing its part as principal partner in developing technology that will provide a 360-degree view of the vehicles surroundings. The car is now equipped with cameras and radar sensors around its entire body in order to detect possible dangers. Result An all-round view of the cars immediate surroundings is a key component in efforts to realise Volvo Cars commitment to a zero vision in terms of serious accidents. The ability to identify and detect hazards in good time will enable drivers to avoid accidents and make driving a Volvo both simpler and safer. Video about Non-Hit Car amp Truck: Youtube Digital learning aid for iPad Assignment Helping Natur amp Kultur to make learning interactive and fun for school children aged 7 to 10, and for their teachers. Challenge When the access and comprehension of digital communication becomes a question of democracy and a critical tool to be a part of society, our school system is one of the first places where children can be given this understanding. Our challenge was to create an interactive educational tool that could work practically in a school environment and cater to different styles of learning. Solution The solution presented was The Isle of Qnodds (Qnoddarnas Vaumlrld ) - a learning aid with a sense of gamification, developed directly for the iPad. In The Isle of Qnodds each pupil has its own login and can furnish their own stump, which is their own home in the program. When the pupils log in they see new exciting tasks that their teacher has activated, everything from exercises with letters to narrative texts or geometry - all in an inspiring and educational setting. Result Today The Isle of Qnodds is used in many schools all over Sweden. HiQs client Natur amp Kultur has received overwhelmingly positive feedback regarding the solution. - a solution for mobile payments Attn. The video above is only available in Swedish. Assignment To create a mobile solution for simple real-time financial transactions between private individuals for Swedens six largest banks in cooperation with Bankgirot. Challenge To free users from bank card readers and the hassle of entering the recipients account number by pioneering a new way of carrying out transactions in real time. The innovative solution also had to work as a common service for Swedens six largest banks, all of which have their own unique security systems and basic processes. Solution In a project that expanded as it progressed, HiQ created the Swish mobile app - a shared service for SEB, Handelsbanken, Nordea, Danske Bank, Laumlnsfoumlrsaumlkringar Bank, and the various branches of Swedbank and Sparbank. Using this service, it is possible to transfer money to individuals with an account in a Swish-affiliated bank in real time. All that is needed for the transaction is the recipients mobile phone number - no card reader or long account numbers are necessary. Payments are approved with the help of a mobile Bank ID, which ensures that the solution is as safe as the banks own individual internet services. Result Millions of Swedes can now easily transfer money in real time via their mobile phones. Swish is a huge success - in its first 2 years it has reached over 2 million users who now use the service to manage various everyday situations, such as paying someone back for a lunch or collecting money to buy someone a joint birthday present. Swish was awarded as Mobile Payment of the Year at the Swedish Mobile Gala 2013. Volvo Roam Delivery Assignment Simplifying the delivery of goods to private individuals together with Volvo Cars. Challenge Many of us have experienced how tricky it can be to have ordered goods delivered, not least since many couriers delivery times dont fit peoples day-to-day lives. Often, one might not be home when the delivery arrives, and having to fetch the goods at the post office is time consuming. Solution HiQ has helped Volvo Cars to develop the technology for the globally acknowledged service Roam Delivery, which allows consumers to have goods delivered straight to the trunk of their connected car. To use the service, the driver of a Volvo simply chooses their car as a delivery address. The delivery company gets access to the cars location, colour and registration number, and to a digital disposable key that is connected to Volvo On Call. With a mobile device, the courier simply unlocks the car on site and leaves the goods in the trunk. The car is then immediately locked and the owner receives a message confirming the delivery. Result Volvo Roam Delivery is both timesaving and hassle-free, for the recipient as well as for the vendor and the courier. Pilot tests of the service showed that 86 per cent experienced the service as timesaving. 92 per cent stated that they would rather have their goods delivered to their cars than to their homes. Facebook for deafblind people Assignment Helping the Swedish Post amp Telecom Authority to make Facebook accessible for everyone, including people suffering from deafblindness and people who are visually impaired. Challenge Not being able to communicate on equal terms leads to people being isolated from society and excluded from social contexts. Today, a lot of our conversations take place online, but for people suffering from deafblindness or who are visually impaired, navigating and communicating on the internet can be difficult. The problem with Facebook is that the interface and the huge amount of information are not compatible with tools such as screen readers and braille displays. The challenge was therefore to create a simpler and more slimmed Facebook, which still presents the user with the most common functionality. Solution Together with PTS, HiQ has developed the service Fejjan foumlr alla, that works just as well on a computer as in a smartphone or tablet. The user-friendly interface connects well with aids such as screen readers, braille displays and magnification software. The service has been developed in close collaboration with the target group and deafblind people, as well as visually impaired, have been testing the service throughout the development project. Fejjan foumlr alla is a clean and slimmed-down version of Facebook in which the most common actions, such as status updates, friends and messages, have been simplified. Result With the service, deafblind people and those who are visually impaired can communicate with family, new acquaintances and others with disabilities on equal terms. Feedback after the launch has been positive, not least for the simplicity of the service. Possible users are the 2,500 individuals who suffer from deafblindness in Sweden, as well as the countrys approximately 100,000 visually impaired. Assignment Helping the S Group in Finland simplify for their customers, by developing and offering the next generation of mobile banking services. Challenge The S Groups in-store bank S-Pankki has over 2,6 million customers in Finland, and service sports in more than 700 of the S Group outlets. S-Pankkis services are foremost aimed at the users of the S Groups customer loyalty card. The challenge was to create an innovative mobile solution that connects all of the S Groups services - customer loyalty bonus, the internet bank as well as other services and benefits, in order to simplify and improve for the customers. Solution HiQ developed a solution called S-mobiili (S Mobile), which is the first mobile bank in the world to be integrated with a retailers customer loyalty program. With the service, S Group customers can manage their bank errands and their loyalty bonus balance at the same time. S-mobiili also offers personalized benefits and discounts as well as an adapted feature for finding service spots. For a complete list of the many smart features, please visit s-mobiili. fi Result S-mobiili simplifies and improves the customers everyday lives, and it received a fantastic welcoming from both experts and users when it was launched. As an example, the service received an honorary mention for Best User Experience in the GrandOne competition. S-mobiili was launched in November 2013 and it was downloaded to nearly 200,000 mobile devices in the first five months alone. S-mobiili has really succeeded in meeting the users expectations and demands, which is proven by an average score of 4 out of 5 stars in reviews of the service. Stena Match Cup Sweden Assignment HiQ is digital partner to Stena Match Cup Sweden, one of Swedens biggest sports events with more than 100,000 visitors yearly. HiQ has a multiannual assignment to help Stena Match Cup Sweden to boost the visitors experience before, during and after the event. This is done by improving and simplifying the digital communication. Challenge Todays connected society creates endless possibilities but also challenges and new needs, thanks to new visitor behaviours and technical platforms. Based on this, the client needs help to develop the event and create new solutions for communication and information - solutions that help visitors to follow the sailing competition and everything else that is happening in connection with the event. Solution Today, mobile and digital solutions are key factors in all sorts of events in the sporting and entertainment worlds. Therefore HiQ has created a new digital platform and external website for the Stena Match Cup, helping the organisation to interact with the visitors and to spread information. This is a step in improving the events digital communication. Result The new digital platform simplifies communications for the event organisation and it also contributes to creating an even better event for Stena Match Cup Swedens 100 000 visitors. The new solution helps the visitors to smoothly access the information that they need. Assignment To help the UK TV station, Channel 4, to create an IPTV service with a new player and portal for YouView - a shared platform for UK public service broadcasters. Challenge The way we watch TV has changed. Todays viewers are used to being able to choose when and how they watch TV. To meet viewers needs, the UKs public service broadcasters and two broadband companies agreed to work together to create YouView - a subscription-free, easy-to-use TV service with more than 70 digital channels, a 7-day catch-up function and a wide variety of TV programmes, films and radio shows on demand. Channel 4 needed to create a completely new player and portal that could be synchronised with this shared solution. Solution HiQ played a key role in the team that created the Channel 4 player and portal for YouView. A critical element of the work was the development of a completely new on-demand player for the new platform, complete with content protection, dynamic advertising and detailed reporting. HiQ also helped Channel 4 to develop a logging system, which is essential for diagnosis and error detection when problems occur. Result With Channel 4s new IPTV service, all the channels viewers can see their favourite programmes when they want to - both live and on-demand on their normal TVs. Assignment To help popular Swedish rock star Dregen to reach out to his fans and media in a way that suits his rocknroll image. Challenge In todays digital world an artist is so much more than someone who writes songs and performs on stage. When the channels are endless, a strategic communication is essential to break through the buzz. Dregen spends much on his time stuck on tour buses all over the world and therefore the solution needs to be simple and easy to use. Its also important with a direct link to the fans so that Dregen can speak to them in person. Solution We created a website and a digital platform which with its raw rocknroll attitude fits the artists image perfectly. The website, based on a flexible, fast, easy-to-use solution, brings Dregens communication to a whole new level and makes it easy for him to work while on the run. Because Dregen takes a very active role in social media this was a given component. Much attention was given to usability and the site as a mobile channel. Result Dregens new website enjoys a great number of visitors and the traffic coming in through mobile channels has increased steadily. The site increases the artists visibility and enables contact with fans and media. Thanks to his new digital platform, Dregen can spend more of his time on song writing and touring the world. Attn. The video above is only available in Finnish. Assignment To create a new and modern mobile service for Fintoto Oy, the group that markets and organizes horse race betting in Finland. Challenge To bring the excitement and drama of horse and harness racing to mobile users. Harness racing is Finlands second-most popular sport in terms of spectator numbers. Annually, some 730,000 spectators attend the Finnish racetracks. In 2012, Tote betting amounted to nearly 250 million euros, half of which comes from online gaming. In step with a growing interest and the arrival of new technology, Fintoto discovered a significant demand for a mobile solution, especially among new players. The service needed to be both modern and easy to use. Solution HiQ developed Toto-mobiili, a service that moves Toto betting and live broadcasts into the smartphones in the players pockets, enabling them to gamble and follow the races anytime and anywhere. The service focuses on the user, combining cutting-edge technology with the joy and excitement of betting. Result Fintotos customers can now easily bet on horse racing and follow live broadcasts in their smartphones. The service simplifies for the users and strengthens Fintotos offer. JAS 39 Gripen flight simulator Assignment Back in the mid-1990s, HiQ was commissioned to develop, manage and support flight simulator training for the JAS39 Gripen multirole fighter aircraft. The simulator was used to train pilots to fly the plane, handle the tactical systems and deal with various emergency situations. Challenge To develop simulators that reflect the advances made and to offer pilots a virtual environment as close to reality as possible, so that on-the-ground training genuinely does resemble the real thing. The aircraft is one of the most technically advanced in the world and the result of one of Swedens biggest ever industrial projects. In many respects, the environment in a simulator is even more complex than that in the plane itself not only the aircraft and its controls, but also the surroundings, operator positions, tactical environment, etc. have to be authentically reproduced. And, of course, the simulators have to be simple for users to operate. Solution We worked on the project using agile methodology, continually taking responsibility for what was most important to the customer and users at every point in time. The assignment included everything from project management to configuration management, safety work, the preparation and specification of requirements, designarchitecture, development, testing, delivery, training and support for users (both technicians and pilots). Result Since the project began, more than 300 pilots have been trained in simulators developed by HiQ. Training in a simulator is very cost-effective and has considerably less impact on the environment. European Athletics indoor championships Assignment To optimise the experience for visitors to the European Athletics indoor championships at Scandinavium in Gothenburg. Challenge When you are watching an athletics competition where several events are taking place at the same time, it can be difficult to follow everything that is happening. It can also be difficult to find your way around the stadium and to make sure you are aware of all the offers that are available. Solution As most people today are online 247, HiQ created an efficient, easy-to-navigate website and the official app for Android and iPhone for the 2013 championships in Gothenburg. These digital platforms made it easier for spectators to follow the competitions by providing background information, live updates and details of other events in connection with the competitions. Result 50,000 unique visitors to the website every day during the championships and 5,200 app downloads during the weekend showed that the digital platform interested and reached a large portion of those attending the event. It was easier to follow the competitions on site and for media, sponsors and competitors to communicate with the events target groups. Stockholm Pride Parade Assignment To carry out the first ever live broadcast of Swedens biggest event - the Stockholm Pride Festival - and to involve participants in the parade with the help of mobile units. Challenge To cope with the huge strain on the internet when more than five hundred thousand people out on the streets of Stockholm are encouraged during an intensive four-hour period to stream pictures for a live TV broadcast. We also wanted to set new records for live streaming during the course of one single day. Openness was the theme of this years Pride Festival, and we wanted to show that technical solutions, such as Channel Q, can contribute to a more open society. Solution We created a four-hour live broadcast with presenter Roger Nordin. Guests on the programme included Danny Saucedo, Babsan and Swedens Minister for European Union Affairs, Birgitta Olsson. By integrating Bambuser with our website it was possible to follow thousands of live pictures from the parade. The camera team worked hard to create a wi-fi carpet to cover the entire the parade route so that everyone could contribute to the fantastic show. The event was broadcast on hiq. se, expressen. se, qx. se and on the large screen in Kungstraumldgaringrden Park in Central Stockholm. Result We pioneered the first ever live broadcast of the Stockholm Pride Parade. We set a new Nordic record for the number of live streams in a day. We set a new visitor record to our website, and tens of thousands of people watched the TV broadcast online. We also succeeded in showing how technology can create a more open society. HiQs market segments Together with innovative and driven clients, HiQ works with simplifying and improving peoples lives using IT. HiQs clients come from a variety of different industries, and they all have something in common - that they want to be at the forefront, developing their businesses and operations through simple and user friendly solutions. We divide our customers, who have primarily Nordic but also global presence, into eight market segments. Mobility and improvements in capacity are becoming ever more important for all clients in all industries. Since 1995, HiQ has a very strong standing in this segment and works with global and market-leading clients. We have extensive technical expertise as well as commercial competence within telecommunications systems. For almost 20 years we have been working closely with suppliers, operators, users and legislative authorities. Our know-how and understanding of the industry creates great value for clients and projects in other lines of business, as almost everyone are dependent on telecom technology and mobility. For example, HiQ helps Microsoft Devices with an efficient and cloud based Internet of Things solution for analytics. The data gathered with the solution aims to improve quality of the mobile devices as well as the end - user experience. The solution makes it possible to forecast trends and modify functionality. Some of our clients in this segment are Ascom Wireless Solutions, Com Hem, Doro, Ericsson, Microsoft Devices, Nokia, Telenor, TDC and Tele2. Vehicles and their components are increasingly becoming connected and able to communicate with each other. This simplifies for people and increases safety on our roads. The technology used in the automotive industry is constantly evolving and there is a great deal of pressure to introduce shorter and more efficient development cycles. Together with innovative clients we are developing active safety systems, such as road trains. Several vehicles travel in a semi-autonomous convoy, which contributes to enhanced safety and reduced fuel consumption. We are also developing a 360-degree field of vision around vehicles. This enables the vehicle to identify hazards and thereby avoid accidents. HiQ is also increasingly involved in projects aimed at developing eco-friendlier vehicles. Technology plays a crucial part in finding effective solutions to reduce emissions and minimise fuel consumption. Some of our clients in this segment are BorgWarner, Scania, Volvo Cars and the Volvo Group. More and more companies are completely dependent on, and open to the possibilites of, mobility and IT. HiQ creates new business offers and solutions that simplify operations. There are a great number of IT-dependent products and support systems that require development capacity and services for testing and quality assurance. The cloud is actively used for the streamlining of processes. HiQ has a strong position in this segment and works with several global players with the aim to simplify and improve. For example, HiQ is strategic partner to Finnair and SAS, with the responsibility to help the airline companies with development, support and operation of business critical crew management systems. Some of our clients in this segment are ABB, Assa Abloy, Atlas Copco, Bombardier, Dekra, Electrolux, Empower, Finnair, Jeppesen Systems, Kuusakoski, Kerava Energy, SAS, SKF and Svevia. The public sector is continually striving to increase service levels and make peoples lives simpler. To do so, the sector is developing IT environments that streamline and strengthen its operations. HiQ has more than ten years experience of working with the development of business-critical IT solutions for public authorities and organizations. The IT development in this segment simplifies for us as citizens, in our contacts and dealings with various authorities. HiQ grows within the healthcare segment and has a number of framework agreements with different municipalities and county councils. Through strategy and technology, we simplify for both healthcare providers and patients - before, during, and after the appointment. We see great possibilities to streamline within this important segment. Some of our clients in this segment are the Swedish Civil Contingencies Agency, the city of Gothenburg, the Traffic Assurance Association, the Swedish Public Employment Service, the Government of Sweden, Region Vaumlstra Goumltaland, the Swedish Post and Telecom Authority, the Swedish Tax Agency, the Stockholm County Council and the Finnish customs. Finance and insurance Today, we want to manage our bank errands simply, quickly and securely - willingly with a mobile device. Because of this, the finance and insurance segment is undergoing great changes. At HiQ, we combine our financial insights with our expertise in mobility to create a very competitive offering within mobile payments and banking services. This is an area in which we continue winning assignments in the Nordic region. Swish, that HiQ developed together with Bankgirot for Swedens six largest banks, is a success with more than two million users. Swish enables people to transfer money in real time, without bank card readers or account numbers. In Finland, the field of mobile banking services is also a large and recognised area of expertise for HiQ. We have, for example, developed the worlds first mobile bank that is connected to a retail chains customer bonus system. Some of our clients in this segment are Bankgirot, Collector, Handelsbanken, Nordea, OP Pohjola Group, the S Group, SEB, Skandiabanken, Swedbank, Varma and Wasa Kredit. Media, gaming and entertainment Thanks to todays technology. this industry is changing rapidly. Today, we can play games or stream high-quality media content almost regardless of where we are. At HiQ we use our know-how and experience from other segments as we help both the experienced players and the start-ups to create the solutions of tomorrow. As an example, online-based TV is growing at a fast pace. HiQ is part of developing a number of services for various TV channels in the Nordic region and elsewhere in Europe. The viewers of today can decide for themselves where, when and how they want to watch TV, for example by using the IPTV service that HiQ has assisted British Channel 4 to develop. In addition, HiQ is involved in developing online gaming. This, too, is a constantly evolving industry, and the Nordic companies are successful on the global gaming market. Some of our clients in this segment are Channel 4, DICE, Fintoto, GTECH, the Gothenburg Symphony Orchestra, King, MTG Radio, MTV 3 Finland, Natur amp Kultur, Rovio, SBS TV, Spotify, Utbildningsradion and Veikkaus. Defence and security We live in a time in which defence and security are becoming more and more important. The view of the world is uncertain and the question of defence is put higher on the agenda - not least is IT security becoming even more important for both companies and authorities. HiQs experience in this area is extensive, as it has been since the start 20 years ago. With our expert know-how, HiQ has been developing training simulators for the JAS 39 Gripen fighter aircraft since the early 1990s. Hundreds of fighter pilots worldwide have been trained in simulators that HiQ helps to develop. HiQ helps the Swedish Defence Materiel Administration (FMV) to simplify tracking of their material using RFID technology - a high-tech collaboration that has won international recognition. Some of our clients in this segment are BAE, the Swedish Defence Materiel Administration (FMV), the Swedish Armed Forces and SAAB. Today, our shopping behaviour is completely different than it was only a few years back. Shopping online is convenient and simple, and online and mobile shopping is constantly breaking new records. HiQ has a thorough understanding of the industry gained over many years, and we supply leading international retail chains with services ranging from concept and design to quality assurance. HiQ works within a broad range that includes e-commerce, mobile commerce, procurement and logistics systems, and tools for marketing and sales. Some of our clients in this segment are ICA, Laumlhikauppa, Stockmann, and Volvohandelns Utvecklings AB. Regardless of where we are, we have become used to being in constant contact with the outside world via mobile phones, laptops and tablets - when at work, on holiday and in our free time. We take it for granted that we have constant access to the services that we feel are important. These might be e-mail, social networking sites, weather reports, mobile banking, or our favourite music, to name just a few. Whatever it might be, the requirements we place on the service with regard to accessibility, privacy, content and usability are great. HiQ provides expertise within mobility and mobile solutions to customers in all market segments and we are highly competent with regard to various technical platforms. We provide consultancy services and work with strategies, applications, usability and quality assurance linked to business benefits in our customers operations. Some examples include a mobile banking application for handling banking errands and a mobile e-commerce platform that allows users to buy goods straight from their mobile phones. Telecommunications involves communication between people and between people and systems. It can also include systems communicating with each other without human involvement. Today, this means that your computer, video game console, mobile, sound system, TV and other devices are able to interact and communicate with the world around them. HiQ has long held a strong position within telecommunications and cooperates with the majority of systems suppliers, mobile phone manufacturers, operators and users in the Nordic region. We are currently witnessing a continued, strong convergence between the Internet, media, entertainment and mobility. Telecom technology is playing a central role here as increasing amounts of information are transmitted with the help of IP. HiQ is active at the very heart of this convergence. One example of this is our development of Dreamparks next-generation IPTV portal. TV distribution over IP networks using a new kind of hybrid box will give viewers access to a broad range of TV channels, the Internet, movie rental services, Facebook and Twitter. In the future, it will even be possible to record programmes directly on mobile phones and tablets. One of the specialist areas in which HiQ has a long-running history of expertise is simulation technology. We are leaders in the Nordic region in software development for simulated environments and work together with many different customers in the field. Ever since the JAS Gripen fighter jet first came into service, HiQ has developed simulators for training pilots. HiQ continues its work on this project as the aircraft and technology continue to be developed. An interesting detail about the JAS Gripen project is that the simulator uses the same system software as the actual fighter jet. HiQ also develops simulators used in the development and testing of new mobile phones and IT solutions for vehicles. Another example is when we helped Forsslund Systems, the Swedish Royal Institute of Technology and Karolinska Institutet to develop an oral surgery simulator. The system is used by dentistry students to practice surgical procedures. Digital communication partner In just a few short years the way in which we communicate has been totally transformed. To meet the new challenges that this presents and the needs it creates, HiQ has assembled a talented team in the form of a digital agency that helps our customers to make their digital communication even more powerful. Todays communication increasingly takes place online and across numerous different platforms. This creates business for HiQ, which is winning more and more assignments as a strategic digital partner. Our expertise in technology, communication and business together with our extensive experience in many market segments and specialist areas means that HiQ is gaining ground in the digital market. The team of art directors, creative directors, copywriters, interaction designers and usability experts in HiQs digital agency frequently works on projects that demand the highest levels of innovation, creativity and technical know-how. The partnership is tailor-made to support the clientacutes business in the best way. In this way HiQ becomes a long-term partner to organisations that regard IT and communication as business critical functions for success. Other collaboration takes the form of a more limited participation in projects, where we interact closely with advertising agencies, etc. IT offers a wealth of opportunities for businesses and the public sector to both improve and simplify what they do. Many companies are highly dependent on their IT systems. Banks and insurance companies are totally dependent on the reliability of the IT systems that manage payments and other transactions, for example. Public sector institutions require support systems to help them keep their operations well organised and to ensure that the services they provide to the public function well. In the case of the Swedish National Tax Board, for example, IT has made it possible to offer better, simpler, more user-friendly services by providing forms, tax declarations and applications online. HiQ develops systems able to meet high demands on accessibility and reliability and that are business-critical for the customers entire operation. Often, these are systems that are required to operate 24-7, such as systems for share trading and case management systems for public authorities. This might also include things like logistical solutions for transport or trading companies. Whatever the case may be, they are always business-critical parts of our customers operations. Gaming and platforms Today there is a veritable ocean of interactive online games. Scratchcards are now available on mobile phones, poker is a success on tablets and there are many new ideas in the pipeline. HiQ works to produce interactive games for betting and sporting games for state-regulated companies. The demand for interactive gaming alternatives is increasing, especially within sports games. We are Nordic leaders in the area and develop and implement platforms for both gaming and the games themselves. HiQ also develops advanced solutions for managing security, accessibility and performance. Our customers include established, well-known Nordic gaming companies and betting agencies, as well as GTECH - the world-leading supplier of IT solutions and services for the gaming and lottery industry. Within finance, HiQ is a specialist in securities trading services. We have successfully managed a very large number of projects and commissions in this area - from strategy and systems to projects and administration. Efficiency is often the most important issue for our customers, e. g. how to make the process faster, how to monitor it better or how to reduce risk. Thanks to HiQs understanding of the flows of the securities industry, we are able to find solutions to these issues. We possess expertise on the entire process - from orders and transactions to clearing and settlement. We provide our customers with insight into the complete process. The finance industry is complex, so it is often possible to focus on and understand only one section of the market. At HiQ, we understand all aspects of the industry. Our comprehensive knowledge allows us to quickly find effective, secure solutions that meet all existing standards and legal requirements, from the time the order is placed to the time the transaction is made and registered. This over-arching capability saves our customers both time and money, allowing them to get up and running quickly and to get to work on new business opportunities. IT for vehicles New vehicles launched onto the market today are packed with IT and technological solutions. This might include an information system for the driver that indicates how the vehicle is performing, a navigation system or an entertainment system for passengers. One area that continues to expand is active safety. Examples of active safety solutions include systems that cause cars to brake automatically when someone or something gets too close and functions that improve driver vision in poor lighting. HiQ helps vehicle manufacturers and sub-suppliers to the automotive industry to develop platforms, systems and services for vehicles. These facilitate communication between different devices within a vehicle and communication to and from a vehicle. We are well positioned within fields like infotainment, active safety, telematics and usability. Another growing area in which HiQ has a long record of experience is mobility and digital channels. In this field, we help Saab Automobile AB with its development of technical platforms for marketing via digital channels and mobile applications, for example. We also develop engine management systems, production systems and diagnostics solutions and have delivered a new production system for a leading global car manufacturer to Brazil and Asia. Through our broad-ranging offerings to the automotive industry, HiQs work saves both money and lives. EXCITING PROJECTS, FUN AT WORK At HiQ we work every day to make peoples lives simpler with the help of IT, while we ourselves develop as individuals and have fun at work. We see technology as a tool for solving complex challenges and making a difference in peoples everyday lives for example, through innovations such as our Swish app for the banking industry and our development of active safety systems for the automotive industry - systems that save lives. At HiQ you work with fascinating assignments for clients across a broad spectrum of industries as part of a network of Swedens brightest and friendliest IT consultants - people who will become your friends, act as a sounding board for your ideas, join your rock band and work out with you in the gym. HiQ gives you the opportunity to constantly develop your skills and learn more about state-of-the-art technology. We have a strong corporate culture at HiQ and work hard to make sure co-workers are healthy, happy and feel that they are developing. We are proud to be ranked among the best employers, and HiQ has been named as Swedens best IT consultant by the business weekly Veckans Affaumlrer on several occasions. If you share our passion for making things simpler and achieving results that help make the world better and more fun, youre most welcome to join us To apply for jobs, please go to hiq. se or hiq. fi. With the insight that diversity strengthens an organisation, the IT company HiQ sees the value in having employees of various backgrounds. During the last 15 years, HiQ has invested greatly in its co-workers. Through the HiCollege concept, with courses and education, employees are given the opportunity to develop and learn, and together create better solutions for HiQs clients. As a result of the efforts and commitment in their employees, HiQ is named one of Swedens 100 Career Companies 2015. AT HiQ, WE PRACTICE WHAT WE PREACH HiQs values - results, responsibility, simplicity amp joy - are our core in every project, every day. Our values create a competitive advantage and a more amusing everyday life for our clients and ourselves. Our task is to deliver what our customers need. Creating results and making our clients satisfied is vital and we follow up our results in order to ensure continued improvement. To serve as a good example and take personal responsibility, both for their team and for the overall result, is a given for our employees. This means delivering the right services at the right time and always aiming to create added value for our clients. We love to tackle difficult challenges and to use advanced technology to simplify peoples everyday life. Simplicity is a guiding principle that makes us creative. For HiQ clients, simplicity means that we are available, quick to respond and not hesitant to proposing improvements. Simplicity is the innovation of our time. At HiQ, we believe in joy and diversity. When having fun at work and being yourself, we believe that you perform better. We have fun working together with our clients, and this shows in the results we create together.
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